Genotyping and primer details


Genotyping and primer details for SALK_030352c - At4g26180
Line ID SALK_030352c
Locus At4g26180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13262082 of line SALK_030352c Chr4:13262082
   
Insertion allele amplicon LBb3 - 31cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31cv: 5'-GTAATGAAGAGCTGCATAGGGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13261342
   
wt amplicon (over InsPos) 46lt - 31cv
wt amplicon start Chr4:13261342
wt amplicon end Chr4:13262279
wt amplicon size 938 bp
Locus specific primer 31cv: 5'-GTAATGAAGAGCTGCATAGGGAAC-3'
2nd locus specific primer 46lt: 5'-TTTGTCGTTGATTGTTTACTCGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11