Genotyping and primer details


Genotyping and primer details for SALK_030639 - At1g76410
Line ID SALK_030639
Locus At1g76410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28669421 of line SALK_030639 Chr1:28669421
   
Insertion allele amplicon LBb3 - 37vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37vi: 5'-TTCTCCAAGAAGCCAATTCTACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28668937
   
wt amplicon (over InsPos) 65vw - 37vi
wt amplicon start Chr1:28668937
wt amplicon end Chr1:28670228
wt amplicon size 1292 bp
Locus specific primer 37vi: 5'-TTCTCCAAGAAGCCAATTCTACAT-3'
2nd locus specific primer 65vw: 5'-CTTTAAGATTTTGGCGATCTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11