Genotyping and primer details


Genotyping and primer details for SALK_033575c - At1g76480
Line ID SALK_033575c
Locus At1g76480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28693426 of line SALK_033575c Chr1:28693426
   
Insertion allele amplicon LBb3 - 34um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34um: 5'-TATTTGATTTGTCCTGAAGCAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28693735
   
wt amplicon (over InsPos) 33vb - 34um
wt amplicon start Chr1:28692819
wt amplicon end Chr1:28693735
wt amplicon size 917 bp
Locus specific primer 34um: 5'-TATTTGATTTGTCCTGAAGCAGTG-3'
2nd locus specific primer 33vb: 5'-AGTTTTTACACACAGAAGCAGGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11