Genotyping and primer details


Genotyping and primer details for SALK_033579c - At4g05450
Line ID SALK_033579c
Locus At4g05450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2759242 of line SALK_033579c Chr4:2759242
   
Insertion allele amplicon LBb3 - 52qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52qz: 5'-TTATAGACCACCCAATTTCTCACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2759691
   
wt amplicon (over InsPos) 09se - 52qz
wt amplicon start Chr4:2758876
wt amplicon end Chr4:2759691
wt amplicon size 816 bp
Locus specific primer 52qz: 5'-TTATAGACCACCCAATTTCTCACC-3'
2nd locus specific primer 09se: 5'-GATCTTTTTGGCATCGTTAATCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11