Genotyping and primer details


Genotyping and primer details for SALK_034924c - At3g15540
Line ID SALK_034924c
Locus At3g15540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5264526 of line SALK_034924c Chr3:5264526
   
Insertion allele amplicon LBb3 - 64tv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64tv: 5'-CAACGAAGCAACATATAAAAAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5263977
   
wt amplicon (over InsPos) 28uu - 64tv
wt amplicon start Chr3:5263977
wt amplicon end Chr3:5265055
wt amplicon size 1079 bp
Locus specific primer 64tv: 5'-CAACGAAGCAACATATAAAAAGCA-3'
2nd locus specific primer 28uu: 5'-TGGCGAATTCGGTATATAGAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11