Genotyping and primer details


Genotyping and primer details for SALK_037416 - At4g16360
Line ID SALK_037416
Locus At4g16360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9245804 of line SALK_037416 Chr4:9245804
   
Insertion allele amplicon LBb3 - 06cn
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06cn: 5'-CATAGATGTCTTTCCCATTCCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9246418
   
wt amplicon (over InsPos) 28dk - 06cn
wt amplicon start Chr4:9245187
wt amplicon end Chr4:9246418
wt amplicon size 1232 bp
Locus specific primer 06cn: 5'-CATAGATGTCTTTCCCATTCCCTA-3'
2nd locus specific primer 28dk: 5'-TTGATGCTATGACAAACAACAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11