Genotyping and primer details


Genotyping and primer details for SALK_038145c - At3g50050
Line ID SALK_038145c
Locus At3g50050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18554495 of line SALK_038145c Chr3:18554495
   
Insertion allele amplicon LBb3 - 24ie
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24ie: 5'-GGTTGAAGTTCTTGCTCAGCTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18554145
   
wt amplicon (over InsPos) 03jw - 24ie
wt amplicon start Chr3:18554145
wt amplicon end Chr3:18554911
wt amplicon size 767 bp
Locus specific primer 24ie: 5'-GGTTGAAGTTCTTGCTCAGCTACT-3'
2nd locus specific primer 03jw: 5'-TTCTCCTGATGTTTGTGGTACTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11