Genotyping and primer details


Genotyping and primer details for SALK_038282c - At3g19040
Line ID SALK_038282c
Locus At3g19040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6571688 of line SALK_038282c Chr3:6571688
   
Insertion allele amplicon LBb3 - 35xb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35xb: 5'-GAAATCGAGACTTACATCCTCTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6571100
   
wt amplicon (over InsPos) 05xf - 35xb
wt amplicon start Chr3:6571100
wt amplicon end Chr3:6572675
wt amplicon size 1576 bp
Locus specific primer 35xb: 5'-GAAATCGAGACTTACATCCTCTGG-3'
2nd locus specific primer 05xf: 5'-ACAATTGTTGTTAAGAGTCTGGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11