Genotyping and primer details


Genotyping and primer details for SALK_038453c - At3g11070
Line ID SALK_038453c
Locus At3g11070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3467728 of line SALK_038453c Chr3:3467728
   
Insertion allele amplicon LBb3 - 42wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42wm: 5'-CATAAACTATAGACCCGTCCCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3468528
   
wt amplicon (over InsPos) 24xa - 42wm
wt amplicon start Chr3:3467373
wt amplicon end Chr3:3468528
wt amplicon size 1156 bp
Locus specific primer 42wm: 5'-CATAAACTATAGACCCGTCCCAAA-3'
2nd locus specific primer 24xa: 5'-AGATCTACAGGCTCACCAAAACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11