Genotyping and primer details


Genotyping and primer details for SALK_038787c - At3g56370
Line ID SALK_038787c
Locus At3g56370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20900259 of line SALK_038787c Chr3:20900259
   
Insertion allele amplicon LBb3 - 07cn
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07cn: 5'-ACGCACTCTGTATCTTGCTACTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20899771
   
wt amplicon (over InsPos) 16dn - 07cn
wt amplicon start Chr3:20899771
wt amplicon end Chr3:20900449
wt amplicon size 679 bp
Locus specific primer 07cn: 5'-ACGCACTCTGTATCTTGCTACTCA-3'
2nd locus specific primer 16dn: 5'-GGTGTAATAGCCATCACGGTACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11