Genotyping and primer details


Genotyping and primer details for SALK_038994c - At1g08230
Line ID SALK_038994c
Locus At1g08230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2585968 of line SALK_038994c Chr1:2585968
   
Insertion allele amplicon LBb3 - 55us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55us: 5'-TCCTAATTTGGATCGAATCCTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2585470
   
wt amplicon (over InsPos) 28vl - 55us
wt amplicon start Chr1:2585470
wt amplicon end Chr1:2586452
wt amplicon size 983 bp
Locus specific primer 55us: 5'-TCCTAATTTGGATCGAATCCTATG-3'
2nd locus specific primer 28vl: 5'-GTACGCCTTCAAGTTCTTAGGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11