Genotyping and primer details


Genotyping and primer details for SALK_039637c - At2g40955
Line ID SALK_039637c
Locus At2g40955 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17093975 of line SALK_039637c Chr2:17093975
   
Insertion allele amplicon LBb3 - 38wj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38wj: 5'-ACATGAAACCTGTGTGTAACCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17094405
   
wt amplicon (over InsPos) 11wy - 38wj
wt amplicon start Chr2:17093414
wt amplicon end Chr2:17094405
wt amplicon size 992 bp
Locus specific primer 38wj: 5'-ACATGAAACCTGTGTGTAACCAAA-3'
2nd locus specific primer 11wy: 5'-AGGGAAGTAAATTTCGGGTTAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11