Genotyping and primer details


Genotyping and primer details for SALK_039817 - At2g42310
Line ID SALK_039817
Locus At2g42310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17625234 of line SALK_039817 Chr2:17625234
   
Insertion allele amplicon LBb3 - 53qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53qz: 5'-AAACAAGCCATGAACTAAGGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17625980
   
wt amplicon (over InsPos) 25sl - 53qz
wt amplicon start Chr2:17624878
wt amplicon end Chr2:17625980
wt amplicon size 1103 bp
Locus specific primer 53qz: 5'-AAACAAGCCATGAACTAAGGAAGA-3'
2nd locus specific primer 25sl: 5'-ATCATCCAATACTGCTTAACCGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11