Genotyping and primer details


Genotyping and primer details for SALK_040636c - At3g10620
Line ID SALK_040636c
Locus At3g10620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3320280 of line SALK_040636c Chr3:3320280
   
Insertion allele amplicon LBb3 - 01nc
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 01nc: 5'-ACAATTCCAAAGATGAAAATTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3320819
   
wt amplicon (over InsPos) 12nv - 01nc
wt amplicon start Chr3:3319751
wt amplicon end Chr3:3320819
wt amplicon size 1069 bp
Locus specific primer 01nc: 5'-ACAATTCCAAAGATGAAAATTGGT-3'
2nd locus specific primer 12nv: 5'-TGGACAAAGAGACACTAGAGATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11