Genotyping and primer details


Genotyping and primer details for SALK_041356c - At3g02490
Line ID SALK_041356c
Locus At3g02490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:515337 of line SALK_041356c Chr3:515337
   
Insertion allele amplicon LBb3 - 59fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 59fr: 5'-TTCAGCAAGGTTCTGTCAGACTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:514572
   
wt amplicon (over InsPos) 03hs - 59fr
wt amplicon start Chr3:514572
wt amplicon end Chr3:515482
wt amplicon size 911 bp
Locus specific primer 59fr: 5'-TTCAGCAAGGTTCTGTCAGACTAA-3'
2nd locus specific primer 03hs: 5'-GTAAATCCTGAGCTTCACTATGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11