Genotyping and primer details


Genotyping and primer details for SALK_041485c - At4g00570
Line ID SALK_041485c
Locus At4g00570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:243429 of line SALK_041485c Chr4:243429
   
Insertion allele amplicon LBb3 - 50dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50dl: 5'-TGATCACAAATAAAAGATGAAGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:242734
   
wt amplicon (over InsPos) 03hf - 50dl
wt amplicon start Chr4:242734
wt amplicon end Chr4:243486
wt amplicon size 753 bp
Locus specific primer 50dl: 5'-TGATCACAAATAAAAGATGAAGCG-3'
2nd locus specific primer 03hf: 5'-AGTTCACTGTGTTGCATTTCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11