Genotyping and primer details


Genotyping and primer details for SALK_042026c - At4g29790
Line ID SALK_042026c
Locus At4g29790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14587489 of line SALK_042026c Chr4:14587489
   
Insertion allele amplicon LBb3 - 34dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34dr: 5'-TTCTTTTTCACATTTAGGGATTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14587819
   
wt amplicon (over InsPos) 11ep - 34dr
wt amplicon start Chr4:14587195
wt amplicon end Chr4:14587819
wt amplicon size 625 bp
Locus specific primer 34dr: 5'-TTCTTTTTCACATTTAGGGATTGG-3'
2nd locus specific primer 11ep: 5'-TGATCAGAATTGTTCTTTGACAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11