Genotyping and primer details


Genotyping and primer details for SALK_044731 - At1g17540
Line ID SALK_044731
Locus At1g17540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6030301 of line SALK_044731 Chr1:6030301
   
Insertion allele amplicon LBb3 - 30eq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30eq: 5'-GTATGGACCTGTGTATAAAGCCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6030753
   
wt amplicon (over InsPos) 15gc - 30eq
wt amplicon start Chr1:6029956
wt amplicon end Chr1:6030753
wt amplicon size 798 bp
Locus specific primer 30eq: 5'-GTATGGACCTGTGTATAAAGCCGT-3'
2nd locus specific primer 15gc: 5'-CTTCTCGGAGTTTCTTCTTCTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11