Genotyping and primer details


Genotyping and primer details for SALK_045957c - At1g77000
Line ID SALK_045957c
Locus At1g77000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28940654 of line SALK_045957c Chr1:28940654
   
Insertion allele amplicon LBb3 - 35dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35dr: 5'-AACAGAGACATACCAAGAGAGGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28941118
   
wt amplicon (over InsPos) 01ft - 35dr
wt amplicon start Chr1:28940243
wt amplicon end Chr1:28941118
wt amplicon size 876 bp
Locus specific primer 35dr: 5'-AACAGAGACATACCAAGAGAGGGA-3'
2nd locus specific primer 01ft: 5'-GTTTACTCTTTGCCTCTTTCCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11