Genotyping and primer details


Genotyping and primer details for SALK_046876c - At1g74040
Line ID SALK_046876c
Locus At1g74040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27842346 of line SALK_046876c Chr1:27842346
   
Insertion allele amplicon LBb3 - 25di
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25di: 5'-GTGGCAATAAACGTATGGATTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27842832
   
wt amplicon (over InsPos) 12ec - 25di
wt amplicon start Chr1:27841884
wt amplicon end Chr1:27842832
wt amplicon size 949 bp
Locus specific primer 25di: 5'-GTGGCAATAAACGTATGGATTCTT-3'
2nd locus specific primer 12ec: 5'-TTTTGTAGTCAAAGCCATTACACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11