Genotyping and primer details


Genotyping and primer details for SALK_047220c - At4g31930
Line ID SALK_047220c
Locus At4g31930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15449974 of line SALK_047220c Chr4:15449974
   
Insertion allele amplicon LBb3 - 15rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15rb: 5'-CAAGATCCAAGTAAATCGGAAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15450415
   
wt amplicon (over InsPos) 18rz - 15rb
wt amplicon start Chr4:15449501
wt amplicon end Chr4:15450415
wt amplicon size 915 bp
Locus specific primer 15rb: 5'-CAAGATCCAAGTAAATCGGAAGAG-3'
2nd locus specific primer 18rz: 5'-GACACAGTTATTAAACATCCCGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11