Genotyping and primer details


Genotyping and primer details for SALK_047914c - At4g14620
Line ID SALK_047914c
Locus At4g14620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8389669 of line SALK_047914c Chr4:8389669
   
Insertion allele amplicon LBb3 - 40vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40vi: 5'-TTTAACGGGAACAATGATATCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8390181
   
wt amplicon (over InsPos) 51vw - 40vi
wt amplicon start Chr4:8389261
wt amplicon end Chr4:8390181
wt amplicon size 921 bp
Locus specific primer 40vi: 5'-TTTAACGGGAACAATGATATCTCC-3'
2nd locus specific primer 51vw: 5'-GAACAGTAAAGCTAATCCCGTGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11