Genotyping and primer details


Genotyping and primer details for SALK_048606c - At2g43280
Line ID SALK_048606c
Locus At2g43280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17990458 of line SALK_048606c Chr2:17990458
   
Insertion allele amplicon LBb3 - 41vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41vi: 5'-GAACAGCAACAACACAGATGATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17990166
   
wt amplicon (over InsPos) 50vw - 41vi
wt amplicon start Chr2:17990166
wt amplicon end Chr2:17991048
wt amplicon size 883 bp
Locus specific primer 41vi: 5'-GAACAGCAACAACACAGATGATTT-3'
2nd locus specific primer 50vw: 5'-CACATTCGTATCAATCTAAAGGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11