Genotyping and primer details


Genotyping and primer details for SALK_049267 - At1g79180
Line ID SALK_049267
Locus At1g79180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29787632 of line SALK_049267 Chr1:29787632
   
Insertion allele amplicon LBb3 - 10en
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10en: 5'-TTCAGAGGAGGAAGAAACAATCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29786826
   
wt amplicon (over InsPos) 20gc - 10en
wt amplicon start Chr1:29786826
wt amplicon end Chr1:29787742
wt amplicon size 917 bp
Locus specific primer 10en: 5'-TTCAGAGGAGGAAGAAACAATCAT-3'
2nd locus specific primer 20gc: 5'-TATAGTGTGCATCTTTTGACCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11