Genotyping and primer details


Genotyping and primer details for SALK_052697c - At3g02950
Line ID SALK_052697c
Locus At3g02950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:665556 of line SALK_052697c Chr3:665556
   
Insertion allele amplicon LBb3 - 16rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16rb: 5'-GAGATTGCTTGAGCTAAGGAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:665888
   
wt amplicon (over InsPos) 17rz - 16rb
wt amplicon start Chr3:664946
wt amplicon end Chr3:665888
wt amplicon size 943 bp
Locus specific primer 16rb: 5'-GAGATTGCTTGAGCTAAGGAAGAA-3'
2nd locus specific primer 17rz: 5'-CAATAAAGCCTATTTGGCTAGTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11