Genotyping and primer details


Genotyping and primer details for SALK_053067c - At2g28110
Line ID SALK_053067c
Locus At2g28110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11978043 of line SALK_053067c Chr2:11978043
   
Insertion allele amplicon LBb3 - 24tg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24tg: 5'-AAAAACATCAGTGGACGCTTCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11978612
   
wt amplicon (over InsPos) 26vb - 24tg
wt amplicon start Chr2:11977528
wt amplicon end Chr2:11978612
wt amplicon size 1085 bp
Locus specific primer 24tg: 5'-AAAAACATCAGTGGACGCTTCTAT-3'
2nd locus specific primer 26vb: 5'-GTCTTTGATGGAGAAATGCTTAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11