Genotyping and primer details

Genotyping and primer details for SALK_053952c - At3g47190
Line ID SALK_053952c
Locus At3g47190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17375184 of line SALK_053952c Chr3:17375184
Insertion allele amplicon LBb3 - 44wm
Locus specific primer 44wm: 5'-TCCATTTGGAGAATACTGGGATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17375869
wt amplicon (over InsPos) 22xa - 44wm
wt amplicon start Chr3:17374596
wt amplicon end Chr3:17375869
wt amplicon size 1274 bp
Locus specific primer 44wm: 5'-TCCATTTGGAGAATACTGGGATAC-3'
2nd locus specific primer 22xa: 5'-AGAATTTGAATGGCTTATAGCGAG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11