Genotyping and primer details


Genotyping and primer details for SALK_056368c - At1g23170
Line ID SALK_056368c
Locus At1g23170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8214419 of line SALK_056368c Chr1:8214419
   
Insertion allele amplicon LBb3 - 44vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44vi: 5'-GCGTAAGAAACCTGATGCTTTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8213998
   
wt amplicon (over InsPos) 48vw - 44vi
wt amplicon start Chr1:8213998
wt amplicon end Chr1:8215277
wt amplicon size 1280 bp
Locus specific primer 44vi: 5'-GCGTAAGAAACCTGATGCTTTAAC-3'
2nd locus specific primer 48vw: 5'-CACCATTACCTCCCTCAGTAAGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11