Genotyping and primer details


Genotyping and primer details for SALK_057821 - At3g08950
Line ID SALK_057821
Locus At3g08950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2727707 of line SALK_057821 Chr3:2727707
   
Insertion allele amplicon LBb3 - 39fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39fx: 5'-TTGAGGTTGGGTCTTATTTTGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2726841
   
wt amplicon (over InsPos) 03hb - 39fx
wt amplicon start Chr3:2726841
wt amplicon end Chr3:2727832
wt amplicon size 992 bp
Locus specific primer 39fx: 5'-TTGAGGTTGGGTCTTATTTTGTTC-3'
2nd locus specific primer 03hb: 5'-CAATCAAACCAAAAACTCTCCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11