Genotyping and primer details


Genotyping and primer details for SALK_058574c - At5g09570
Line ID SALK_058574c
Locus At5g09570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2971669 of line SALK_058574c Chr5:2971669
   
Insertion allele amplicon LBb3 - 25ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25ke: 5'-GGAAGATCTAGCTACCGTCCTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2971017
   
wt amplicon (over InsPos) 05ll - 25ke
wt amplicon start Chr5:2971017
wt amplicon end Chr5:2971932
wt amplicon size 916 bp
Locus specific primer 25ke: 5'-GGAAGATCTAGCTACCGTCCTTCT-3'
2nd locus specific primer 05ll: 5'-CACTCAGCATGTCCATGTAGAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11