Genotyping and primer details


Genotyping and primer details for SALK_059398 - At1g16160
Line ID SALK_059398
Locus At1g16160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5537797 of line SALK_059398 Chr1:5537797
   
Insertion allele amplicon LBb3 - 35uv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35uv: 5'-ATATGGGCCTCTAAATCTTCAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5538220
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11