Genotyping and primer details


Genotyping and primer details for SALK_061038c - At3g46620
Line ID SALK_061038c
Locus At3g46620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17179867 of line SALK_061038c Chr3:17179867
   
Insertion allele amplicon LBb3 - 23td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23td: 5'-ACTCAAATCTACACACAGGACACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17179250
   
wt amplicon (over InsPos) 03ua - 23td
wt amplicon start Chr3:17179250
wt amplicon end Chr3:17180287
wt amplicon size 1038 bp
Locus specific primer 23td: 5'-ACTCAAATCTACACACAGGACACG-3'
2nd locus specific primer 03ua: 5'-AAGGTAACAGTTTTCACATTTGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11