Genotyping and primer details


Genotyping and primer details for SALK_061079c - At3g56850
Line ID SALK_061079c
Locus At3g56850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21046701 of line SALK_061079c Chr3:21046701
   
Insertion allele amplicon LBb3 - 70sa
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 70sa: 5'-TGGTTTAACTTCAAGCTTGGCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21046120
   
wt amplicon (over InsPos) 06sx - 70sa
wt amplicon start Chr3:21046120
wt amplicon end Chr3:21047232
wt amplicon size 1113 bp
Locus specific primer 70sa: 5'-TGGTTTAACTTCAAGCTTGGCTAT-3'
2nd locus specific primer 06sx: 5'-AGAGAAGACTGTAGAGAGGAGGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11