Genotyping and primer details


Genotyping and primer details for SALK_063593c - At5g64640
Line ID SALK_063593c
Locus At5g64640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25838220 of line SALK_063593c Chr5:25838220
   
Insertion allele amplicon LBb3 - 13ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13ii: 5'-GTAAAAACGGAGGGAAAGATTGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25837694
   
Insertion allele amplicon LBb3 - 33gz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33gz: 5'-CATGATGTTATCGTTCGACAGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25837527
   
wt amplicon (over InsPos) 07ir - 33gz
wt amplicon start Chr5:25837527
wt amplicon end Chr5:25838554
wt amplicon size 1028 bp
Locus specific primer 33gz: 5'-CATGATGTTATCGTTCGACAGTTT-3'
2nd locus specific primer 07ir: 5'-TGGATTCGACATTGTTTGTAGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11