Genotyping and primer details


Genotyping and primer details for SALK_064185 - At3g42730
Line ID SALK_064185
Locus At3g42730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:14845799 of line SALK_064185 Chr3:14845799
   
Insertion allele amplicon LBb3 - 75xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 75xo: 5'-TGGTTTTCTTCTGGGTGAAATAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:14846858
   
Insertion allele amplicon R204 - CF96
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CF96: 5'-AATTAGAAAGCGAAACGAAATTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:14845039
   
wt amplicon (over InsPos) CF97 - CF96
wt amplicon start Chr3:14845039
wt amplicon end Chr3:14845990
wt amplicon size 952 bp
Locus specific primer CF96: 5'-AATTAGAAAGCGAAACGAAATTCA-3'
2nd locus specific primer CF97: 5'-TAACATCCTCCACGTCTTTCTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11