Genotyping and primer details


Genotyping and primer details for SALK_064700c - At4g28470
Line ID SALK_064700c
Locus At4g28470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14068094 of line SALK_064700c Chr4:14068094
   
Insertion allele amplicon LBb3 - 47sf
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47sf: 5'-ACAACACCCAAGTTTAGGATTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14067640
   
wt amplicon (over InsPos) 14tl - 47sf
wt amplicon start Chr4:14067640
wt amplicon end Chr4:14068651
wt amplicon size 1012 bp
Locus specific primer 47sf: 5'-ACAACACCCAAGTTTAGGATTCAC-3'
2nd locus specific primer 14tl: 5'-CAAAGACGTTCAATGAGAAAATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11