Genotyping and primer details


Genotyping and primer details for SALK_064776c - At3g14000
Line ID SALK_064776c
Locus At3g14000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4631180 of line SALK_064776c Chr3:4631180
   
Insertion allele amplicon LBb3 - 46lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46lu: 5'-TCGACAAGGTCATGGAATTATACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4631906
   
wt amplicon (over InsPos) 21mu - 46lu
wt amplicon start Chr3:4631032
wt amplicon end Chr3:4631906
wt amplicon size 875 bp
Locus specific primer 46lu: 5'-TCGACAAGGTCATGGAATTATACA-3'
2nd locus specific primer 21mu: 5'-AAGCACATTCATTATCTGAATCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11