Genotyping and primer details


Genotyping and primer details for SALK_065067c - At5g42010
Line ID SALK_065067c
Locus At5g42010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16804728 of line SALK_065067c Chr5:16804728
   
Insertion allele amplicon LBb3 - 19oz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19oz: 5'-GACTTCTCTCAACCCAACAAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16804338
   
wt amplicon (over InsPos) 01qh - 19oz
wt amplicon start Chr5:16804338
wt amplicon end Chr5:16805079
wt amplicon size 742 bp
Locus specific primer 19oz: 5'-GACTTCTCTCAACCCAACAAAAAG-3'
2nd locus specific primer 01qh: 5'-ATGAAATGATATCTCCACGAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11