Genotyping and primer details


Genotyping and primer details for SALK_065334c - At5g13640
Line ID SALK_065334c
Locus At5g13640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4396904 of line SALK_065334c Chr5:4396904
   
Insertion allele amplicon LBb3 - 76hw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 76hw: 5'-CAAGATCCCAAATACTGGTCAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4396477
   
wt amplicon (over InsPos) 12jm - 76hw
wt amplicon start Chr5:4396477
wt amplicon end Chr5:4397245
wt amplicon size 769 bp
Locus specific primer 76hw: 5'-CAAGATCCCAAATACTGGTCAAAT-3'
2nd locus specific primer 12jm: 5'-GCTGACAGGACAGCTTAAAGAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11