Genotyping and primer details


Genotyping and primer details for SALK_066515c - At4g34240
Line ID SALK_066515c
Locus At4g34240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16390048 of line SALK_066515c Chr4:16390048
   
Insertion allele amplicon LBb3 - 70kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 70kh: 5'-CTTCTACACGTCTTCTCTTCCGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16389675
   
wt amplicon (over InsPos) 10lz - 70kh
wt amplicon start Chr4:16389675
wt amplicon end Chr4:16390632
wt amplicon size 958 bp
Locus specific primer 70kh: 5'-CTTCTACACGTCTTCTCTTCCGAC-3'
2nd locus specific primer 10lz: 5'-GATGCATTTCTCCTTCTCATCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11