Genotyping and primer details


Genotyping and primer details for SALK_067236c - At2g34440
Line ID SALK_067236c
Locus At2g34440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14527405 of line SALK_067236c Chr2:14527405
   
Insertion allele amplicon LBb3 - 13rr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13rr: 5'-ACCAACATATTCACTTTCACAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14526879
   
wt amplicon (over InsPos) 04sx - 13rr
wt amplicon start Chr2:14526879
wt amplicon end Chr2:14527878
wt amplicon size 1000 bp
Locus specific primer 13rr: 5'-ACCAACATATTCACTTTCACAGCA-3'
2nd locus specific primer 04sx: 5'-AAGCCTTCTAGGATGGGTTTGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11