Genotyping and primer details


Genotyping and primer details for SALK_067859c - At3g23790
Line ID SALK_067859c
Locus At3g23790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8575767 of line SALK_067859c Chr3:8575767
   
Insertion allele amplicon LBb3 - 34gz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34gz: 5'-GAAAGGTTCCATTACTCATCCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8576348
   
wt amplicon (over InsPos) 11il - 34gz
wt amplicon start Chr3:8575231
wt amplicon end Chr3:8576348
wt amplicon size 1118 bp
Locus specific primer 34gz: 5'-GAAAGGTTCCATTACTCATCCAAA-3'
2nd locus specific primer 11il: 5'-AGCAACTTCATCACGATAACCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11