Genotyping and primer details


Genotyping and primer details for SALK_068498c - At4g25990
Line ID SALK_068498c
Locus At4g25990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13192386 of line SALK_068498c Chr4:13192386
   
Insertion allele amplicon LBb3 - 31jr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31jr: 5'-CAAAGAGCTCTGAAGTTACGGACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13192726
   
wt amplicon (over InsPos) 06lz - 31jr
wt amplicon start Chr4:13191991
wt amplicon end Chr4:13192726
wt amplicon size 736 bp
Locus specific primer 31jr: 5'-CAAAGAGCTCTGAAGTTACGGACT-3'
2nd locus specific primer 06lz: 5'-CTGTTGATGAGAGACAACCACAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11