Genotyping and primer details


Genotyping and primer details for SALK_068598 - At1g20460
Line ID SALK_068598
Locus At1g20460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7092808 of line SALK_068598 Chr1:7092808
   
Insertion allele amplicon LBb3 - 24td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24td: 5'-CACTCCATTTTTAAGTTCAGGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7092491
   
wt amplicon (over InsPos) 17ua - 24td
wt amplicon start Chr1:7092491
wt amplicon end Chr1:7093417
wt amplicon size 927 bp
Locus specific primer 24td: 5'-CACTCCATTTTTAAGTTCAGGAGC-3'
2nd locus specific primer 17ua: 5'-AGATTGGTTTTATTGTTTCATGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11