Genotyping and primer details


Genotyping and primer details for SALK_069060c - At5g53760
Line ID SALK_069060c
Locus At5g53760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21825597 of line SALK_069060c Chr5:21825597
   
Insertion allele amplicon LBb3 - 07cl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07cl: 5'-ACTTCTGGATAGCGATTATTCCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21825069
   
wt amplicon (over InsPos) 15cu - 07cl
wt amplicon start Chr5:21825069
wt amplicon end Chr5:21825886
wt amplicon size 818 bp
Locus specific primer 07cl: 5'-ACTTCTGGATAGCGATTATTCCTG-3'
2nd locus specific primer 15cu: 5'-TTATAGTTCGTTCCCATCTGGAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11