Genotyping and primer details


Genotyping and primer details for SALK_069400c - At1g26190
Line ID SALK_069400c
Locus At1g26190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9059267 of line SALK_069400c Chr1:9059267
   
Insertion allele amplicon LBb3 - 02nc
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02nc: 5'-GTGTGTCTGTTACTGGTGGAGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9059606
   
wt amplicon (over InsPos) 03oj - 02nc
wt amplicon start Chr1:9058879
wt amplicon end Chr1:9059606
wt amplicon size 728 bp
Locus specific primer 02nc: 5'-GTGTGTCTGTTACTGGTGGAGTTC-3'
2nd locus specific primer 03oj: 5'-CAGAACCACAGTTATTCACCAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11