Genotyping and primer details


Genotyping and primer details for SALK_069622c - At5g65030
Line ID SALK_069622c
Locus At5g65030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25976003 of line SALK_069622c Chr5:25976003
   
Insertion allele amplicon LBb3 - 25ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25ty: 5'-CTGCAATGGATAATCTATGACCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25976541
   
wt amplicon (over InsPos) 20uu - 25ty
wt amplicon start Chr5:25975555
wt amplicon end Chr5:25976541
wt amplicon size 987 bp
Locus specific primer 25ty: 5'-CTGCAATGGATAATCTATGACCAC-3'
2nd locus specific primer 20uu: 5'-AACTGTTTCAACTTTCGAGTTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11