Genotyping and primer details


Genotyping and primer details for SALK_069686 - At5g05710
Line ID SALK_069686
Locus At5g05710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1715972 of line SALK_069686 Chr5:1715972
   
Insertion allele amplicon LBb3 - 39fp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39fp: 5'-ATCTTCTTTCTCCTTCTCCGAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1716290
   
wt amplicon (over InsPos) 14go - 39fp
wt amplicon start Chr5:1715571
wt amplicon end Chr5:1716290
wt amplicon size 720 bp
Locus specific primer 39fp: 5'-ATCTTCTTTCTCCTTCTCCGAATC-3'
2nd locus specific primer 14go: 5'-ATCATCGATTACGCTTTCTCTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11