Genotyping and primer details


Genotyping and primer details for SALK_070099 - At3g19240
Line ID SALK_070099
Locus At3g19240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6665782 of line SALK_070099 Chr3:6665782
   
Insertion allele amplicon LBb3 - 12gj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12gj: 5'-TTGTTACCGGAGAAGGAGAAGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6666347
   
wt amplicon (over InsPos) 49xt - 12gj
wt amplicon start Chr3:6664831
wt amplicon end Chr3:6666347
wt amplicon size 1517 bp
Locus specific primer 12gj: 5'-TTGTTACCGGAGAAGGAGAAGTTA-3'
2nd locus specific primer 49xt: 5'-AGGTTAAAGCTAGGGTTTCTACGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11