Genotyping and primer details


Genotyping and primer details for SALK_074525 - At2g35510
Line ID SALK_074525
Locus At2g35510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14917161 of line SALK_074525 Chr2:14917161
   
Insertion allele amplicon LBb3 - 09ei
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09ei: 5'-TTCTCTGCATATGGTTTTAGCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14916161
   
wt amplicon (over InsPos) 24gc - 09ei
wt amplicon start Chr2:14916161
wt amplicon end Chr2:14917275
wt amplicon size 1115 bp
Locus specific primer 09ei: 5'-TTCTCTGCATATGGTTTTAGCCTT-3'
2nd locus specific primer 24gc: 5'-ATGTTGCTTGTTTGTATGAAGCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11